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Search result for '29 '. Viewing records 851 to 900 of 972 hits.



N102861 Name: AT.302 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102862 Name: AT.305 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102863 Name: AT.306 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102864 Name: AT.310 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102865 Name: AT.312 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102866 Name: AT.322 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102867 Name: AT.341 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102868 Name: AT.363 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102869 Name: AT.456 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102870 Name: AT.472 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102871 Name: AT.477 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102872 Name: AT.574 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102873 Name: AT.578 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102874 Name: AT.604 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102875 Name: AT.617 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102876 Name: AT.3003 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102877 Name: AT.3010 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102878 Name: AT.3018 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102879 Name: AT.3027 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102880 Name: AT.3029 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102881 Name: AT.3059 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102882 Name: AT.3068 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102883 Name: AT.3080 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102884 Name: AT.3086 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102885 Name: AT.3088 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102886 Name: AT.3090 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102887 Name: AT.3095 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102888 Name: AT.3102 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102889 Name: AT.3107 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102890 Name: AT.3109 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102891 Name: AT.3113 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102892 Name: AT.3115 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102893 Name: AT.3116 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102894 Name: AT.3126 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102895 Name: AT.3136 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102896 Name: AT.3139 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102897 Name: AT.3151 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102898 Name: AT.3152 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102899 Name: AT.3153 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102900 Name: AT.3156 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102901 Name: AT.3158 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102902 Name: AT.3169 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102903 Name: AT.3181 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102904 Name: AT.3182 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102905 Name: AT.3190 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102906 Name: AT.3194 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102907 Name: AT.3204 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102908 Name: AT.3205 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102909 Name: AT.3216 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
N102910 Name: AT.3218 Price: £11.00
Donor
  • John Innes Centre Caroline Dean
Stock type: individual line
Material type: seed


Description
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.
Phenotype
Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.